The M27 at Portsmouth has now been reopened in both directions after an accident involving a lorry and two cars.
Traffic is moving slowly between Junction 12 with the M275 and Junction 11 for Fareham.
Congestion remains between Chichester and Portsmouth and motorists have been advised that they could face delays of up to one hour.
Two people have suffered non-serious injuries in the incident, and are being taken to Queen Alexandra Hospital.
South Central Ambulance Service have sent two ambulances, a hazardous area response team and an air ambulance to the scene.
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