Jack Whatmough and Michael Drennan have been ruled out of tonight’s visit of Northampton.
And the injured duo have also had to pull out of international duty.
Whatmough damaged a hamstring against York at the weekend, ruling him out for at least the next two matches.
He also misses out on the England under-19s, who play three qualifying matches for the European Championships from October 10-15.
The 18-year-old had stepped up from the standby list to make the squad – but has now dropped out himself.
Meanwhile, Drennan has suffered a kick to a calf, having initially thought he’d suffered cramp at Bootham Crescent on Saturday.
As a result, he is absent from the Republic of Ireland’s under-21 friendly in Norway on Thursday.
And, of course, Andy Awford cannot consider the pair for Pompey in tonight’s Johnstone’s Paint Trophy clash against Northampton at Fratton Park.
Awford said: ‘Jack has a hamstring and won’t be able to go with England.
‘Originally, he was on stand-by for the under-19s, then got a call-up to the main squad.
‘They flew out of Sunday for three qualifiers and he would have been unavailable for us for two weeks.
‘As it is, he is definitely out for two matches, possibly more.
‘We won’t know the full extent of it just yet.
‘With Michael Drennan we thought it was cramp, but he has actually got a kick on the calf so he is unavailable tonight and rated as 50/50 for Saturday.
‘He had been called up for the Republic of Ireland under-21s to play midweek, but he’s not going to be fit.’